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KMID : 0613820070170101426
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Journal of Life Science
2007 Volume.17 No. 10 p.1426 ~ p.1433
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Analysis of Antibiotic Resistant Patterns in Conjugant and Transformant of Three ESBL gene Harboring Klebsiella pneumoniae
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Kim Yun-Tae
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Abstract
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To investigate the antibiotic resistant patterns of the bacteria producing ESBL, we isolated one organism of Klebsiella pneumoniae from a clinical laboratory in Busan. The organism that produces ESBL gene was detected by double disk synergy test and the presence of three ESBL genes (TEM-1, SHV-12, CTX-M-15) was confirmed by polymerase chain reaction and DNA sequencing analysis. To analyse the characteristics of three ESBL genes, we performed transconjugation, transformation and cloning experiment with the organism. The MIC of Klebsiella pneumoniae was revealed that ceftazidime, cefotaxime and ceftriaxone were 256 μg/ml, 128 μg/ml and 128 μg/ml respectively. The MIC of conjugant (E. coli RG176Naⓡ) was revealed that ceftazidime, cefotaxime and ceftriaxone were 256 μg/ml, 64 μg/ml and 128 μg/ml respectively. The MIC of transformant (E. coli DH5α) was revealed that ceftazidime, cefotaxime and ceftriaxone were 128 μg /ml, 32 μg/ml, and 32 μg/ml respectively. The MIC of cloned organism of SHV-12 gene (E. coli DH5α) was revealed that ceftazidime, cefotaxime and ceftriaxone were 128 μg/ml, 8 μg/ml, and 32 μg/ml respectively. The results indicated that MIC of conjugant was higher than MIC of transformant and also SHV-12 gene were not resistant against cefotaxime antibiotic.
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KEYWORD
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Klebsiella pneumoniae, ESBL gene, TEM-1, SHV-12, CTX-M-15
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